In this RP-HPLC method, the separation was performed using BDS Hypersil 5µ C18 (150 X 4.6 mm) column at room temperature by using methanol: 2% of Glacial acetic acid in water (80:20 v/v
Ascentis® Express C18, 2.7 μm HPLC Column 2.7 μm particle size, L × I.D. 5 cm × 3 mm; find Supelco-53811U MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich
1-4mL Autosampler Vials for HPLC, UPLC, GC . 16mm, 25mm Test Tubes for Water Analysis 13mm Routine Glass Vial in Stock Technical Grade Position:
18th European Symposium on Radiopharmacy and Radiopharmaceuticals. EJNMMI Radiopharmacy and Chemistry, 2016. Uday Bhonsle
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Apr 18, 2007 · For the size-exclusion chromatography (SE-HPLC), a 4 μm Zorbax™ GF-250 column (4.6 × 250 mm), (Aijiren, USA) was employed. The mobile phase was an aqueous solution of 20 mM phosphate buffer with pH adjusted to 6.0 with sodium hydroxide. Flow rate was set at 0.4ml/min and analysis was carried out at room temperature.
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1.5 ml 4 ml chromatography autosampler vial. 1-4ml sample vial are widely used in chromatographic analysis. 1-4ml chromatography autosample vial include screw top vial, snap-top vial and crimp-top vial. Those vial can be used for Waters, Shimadzu, Thermo Fisher, Scientifific, DIONEX, Techcomp, Varian, Perkinelmer and CTC Analystics.
Oct 04, 2017 · Prior to HPLC injection, crude product underwent SPE (tC18 Light, 4mL DI water dilution, 6 mL DI water rinse, eluted with 700 μL ethanol) and eluate was diluted in 1 mL SWI. For each condition tested, 1/6 th of the post-SPE product was injected onto the semi-preparative HPLC system.
Sigma–Aldrich (St. Louis, MO). 6:2 Cl-PFAES was obtained from Shanghai Synica Co., Ltd. (Shanghai, China). HPLC grade methanol and ammonium hydroxide (NH4OH, 50%, v/v) were purchased from J. T. Baker (Phillipsburg, NJ) and Alfa Aesar (Ward Hill, MA), respectively. HPLC grade acetic acid (CH3CO2H) and ammonium
Oct 31, 2019 · HPLC-grade methanol and acetonitrile were purchased from Fisher Chemicals (Pittsburgh, PA, USA). Formic acid (FA, 99%) and trifluoroacetic acid (TFA, 99%) were obtained from Sigma-Aldrich (St Louis, MO, USA).
Eflornithine hydrochloride and 200mg vials (Ornidyl ®) were gifts from Wintac Limited, Bangalore (India). All reagents applied as solvents were analytical grade and obtained from Sigma-Aldrich (USA). Solvent utilized throughout the project was 95% ethanol. Water was always distilled. 2.2. Instrumetation
Jan 01, 2018 · Stage Tips, Empore™ C18-embeded membrane (Sigma-Aldrich - catalog #2215-C18): Punch a small hole through the four layers of Empore C18 material discs and mount it at the narrow end of an unautoclaved 200μL pipet tip. Methanol, HPLC grade. 80% Acetonitrile (ACN), 5% Formic acid (FA). 5% Formic acid. 5% Acetonitrile (ACN), 5% Formic acid (FA).
Aug 01, 2009 · The peptides were resolubilized in 0.1% TFA and purified by reverse-phase HPLC Ultimate 3000 (Dionex, Sunnyvale, CA) monitoring peptide elution at 230 nm using a Vydac C 18 column (10 × 250 mm, 10μm resin) with a linear gradient of 0.1% TFA in water (v/v) and 0.85% TFA in 50% acetonitrile (v/v) at a flow rate of 4mL/minute over 60 minutes
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