First, the XXT solution was prepared by thawing the XTT labeling reagent and the electron-coupling reagent, respectively in a water bath at 37oC. Mix each vial thoroughly to obtain a clear solution. Then XTT labeling mixture was prepared by mixing 4ml of XTT labeling reagent with 80ul of electron-coupling reagent, to prepare the XTT labeling
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All sample extracts were completely dried at 40°C under nitrogen in a Zymark TurboVap. Samples were reconstituted in 150 µL mobile phase A:B 70:30 (v/v), vortexed 15 sec prior to centrifugation at 4°C, 4000 × g for 5 min and transferred to autosampler vials containing 200 µl glass inserts. 25 µl was injected onto the LCMSMS instrument.
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