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Pak C18 extraction were reconstituted with 4mL of ethyl acetate/methanol/sulphuric acid (80:20:0.06, v/v/v) and incubated at 558C for 2h. The samples were then neutralized with 60mL of 1M NaOH and evaporated to dryness under a stream of nitrogen. The residues were reconstituted in 1mL of sodium phosphate buffer (0.2M, pH 7) and 250mLof 5% K 2CO
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